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VS-1007 - Use default names for VQSR-Lite generated code. #8412

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Jul 14, 2023
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4 changes: 2 additions & 2 deletions scripts/variantstore/wdl/GvsCreateFilterSet.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -293,7 +293,7 @@ task ExtractFilterTask {
>>>

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
memory: "7 GB"
disks: "local-disk 10 HDD"
bootDiskSizeGb: 15
Expand Down Expand Up @@ -355,7 +355,7 @@ task PopulateFilterSetSites {
>>>

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
memory: "3500 MB"
disks: "local-disk 200 HDD"
bootDiskSizeGb: 15
Expand Down
2 changes: 1 addition & 1 deletion scripts/variantstore/wdl/GvsExtractCallset.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -354,7 +354,7 @@ task ExtractTask {
echo ~{interval_index},${OUTPUT_FILE_DEST},${OUTPUT_FILE_BYTES},${OUTPUT_FILE_INDEX_DEST},${OUTPUT_FILE_INDEX_BYTES} >> manifest.txt
>>>
runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
memory: "12 GB"
disks: "local-disk 150 HDD"
bootDiskSizeGb: 15
Expand Down
2 changes: 1 addition & 1 deletion scripts/variantstore/wdl/GvsImportGenomes.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -323,7 +323,7 @@ task LoadData {
>>>

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
maxRetries: load_data_maxretries
memory: "3.75 GB"
disks: "local-disk 50 HDD"
Expand Down
4 changes: 2 additions & 2 deletions scripts/variantstore/wdl/GvsJointVariantCalling.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -38,8 +38,8 @@ workflow GvsJointVariantCalling {
Int SNP_VQSR_CLASSIC_max_gaussians_override = 6
Int SNP_VQSR_CLASSIC_mem_gb_override = ""
}
# This is the most updated snapshot of the code as of July 2, 2023
File gatk_override = "gs://gvs_quickstart_storage/jars/gatk-package-4.2.0.0-724-gf478fb2-SNAPSHOT-local.jar"
# This is the most updated snapshot of the code as of July 12, 2023
File gatk_override = "gs://gvs_quickstart_storage/jars/gatk-package-4.2.0.0-727-g950da0b-SNAPSHOT-local.jar"
File interval_list = "gs://gcp-public-data--broad-references/hg38/v0/wgs_calling_regions.hg38.noCentromeres.noTelomeres.interval_list"

File interval_weights_bed = "gs://broad-public-datasets/gvs/weights/gvs_vet_weights_1kb.bed"
Expand Down
2 changes: 1 addition & 1 deletion scripts/variantstore/wdl/GvsQuickstartIntegration.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -34,7 +34,7 @@ workflow GvsQuickstartIntegration {
}

File full_interval_list = "gs://gcp-public-data--broad-references/hg38/v0/wgs_calling_regions.hg38.noCentromeres.noTelomeres.interval_list"
File expected_output_prefix = "gs://gvs-internal-quickstart/integration/2023-06-06-quicker/"
File expected_output_prefix = "gs://gvs-internal-quickstart/integration/2023-07-12-quicker/"

call FilterIntervalListChromosomes {
input:
Expand Down
6 changes: 3 additions & 3 deletions scripts/variantstore/wdl/GvsUtils.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -41,7 +41,7 @@ task MergeVCFs {
}

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
preemptible: select_first([preemptible_tries, 3])
memory: "3 GiB"
disks: "local-disk ~{disk_size} HDD"
Expand Down Expand Up @@ -127,7 +127,7 @@ task SplitIntervals {
>>>

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
bootDiskSizeGb: 15
memory: "~{disk_memory} GB"
disks: "local-disk ~{disk_size} HDD"
Expand Down Expand Up @@ -934,7 +934,7 @@ task PopulateFilterSetInfo {
>>>

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
memory: "3500 MB"
disks: "local-disk 250 HDD"
bootDiskSizeGb: 15
Expand Down
4 changes: 2 additions & 2 deletions scripts/variantstore/wdl/GvsVQSRClassic.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -362,7 +362,7 @@ task GatherTranches {
bootDiskSizeGb: 15
disks: "local-disk " + disk_size + " HDD"
preemptible: 1
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
}

output {
Expand Down Expand Up @@ -565,7 +565,7 @@ task PopulateFilterSetTranches {
>>>

runtime {
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_02_e90d90e00615dcbd9a71d4301fdc04fe2fe155fc"
docker: "us.gcr.io/broad-dsde-methods/broad-gatk-snapshots:varstore_2023_07_12"
memory: "3500 MB"
disks: "local-disk 200 HDD"
bootDiskSizeGb: 15
Expand Down
Original file line number Diff line number Diff line change
@@ -1,18 +1,13 @@
package org.broadinstitute.hellbender.tools.gvs.common;

import htsjdk.samtools.SAMSequenceDictionary;
import htsjdk.variant.variantcontext.Allele;
import htsjdk.variant.variantcontext.VariantContext;
import htsjdk.variant.vcf.*;
import htsjdk.variant.vcf.VCFConstants;
import org.apache.commons.lang.StringUtils;
import org.broadinstitute.hellbender.utils.genotyper.IndexedAlleleList;
import org.broadinstitute.hellbender.utils.variant.GATKVCFConstants;
import org.broadinstitute.hellbender.utils.variant.GATKVCFHeaderLines;

import java.util.ArrayList;
import java.util.HashSet;
import java.util.List;
import java.util.Set;
import java.util.stream.Collectors;

//TODO rename this or get rid of it. a place holder for now
Expand Down Expand Up @@ -53,92 +48,6 @@ public static String getGTString(final VariantContext variant) {
return StringUtils.join(gsStrings, separator);
}

public static VCFHeader generateVcfHeader(Set<String> sampleNames,
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Migrated to ExtractFeaturesEngine

final SAMSequenceDictionary sequenceDictionary,
final Set<VCFHeaderLine> extraHeaders) {
final Set<VCFHeaderLine> headerLines = new HashSet<>();

headerLines.addAll( getEvoquerVcfHeaderLines() );
headerLines.addAll( extraHeaders );
// headerLines.addAll( defaultHeaderLines );

final VCFHeader header = new VCFHeader(headerLines, sampleNames);
header.setSequenceDictionary(sequenceDictionary);

return header;
}

// TODO is this specific for cohort extract? if so name it such
public static Set<VCFHeaderLine> getEvoquerVcfHeaderLines() {
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Migrated to ExtractFeaturesEngine

final Set<VCFHeaderLine> headerLines = new HashSet<>();

// TODO: Get a list of all possible values here so that we can make sure they're in the VCF Header!

// Add standard VCF fields first:
VCFStandardHeaderLines.addStandardInfoLines( headerLines, true,
VCFConstants.STRAND_BIAS_KEY,
VCFConstants.DEPTH_KEY,
VCFConstants.RMS_MAPPING_QUALITY_KEY,
VCFConstants.ALLELE_COUNT_KEY,
VCFConstants.ALLELE_FREQUENCY_KEY,
VCFConstants.ALLELE_NUMBER_KEY,
VCFConstants.END_KEY
);

VCFStandardHeaderLines.addStandardFormatLines(headerLines, true,
VCFConstants.GENOTYPE_KEY,
VCFConstants.GENOTYPE_QUALITY_KEY,
VCFConstants.DEPTH_KEY,
VCFConstants.GENOTYPE_PL_KEY,
VCFConstants.GENOTYPE_ALLELE_DEPTHS
);

headerLines.add(GATKVCFHeaderLines.getFormatLine(GATKVCFConstants.REFERENCE_GENOTYPE_QUALITY));

headerLines.add(GATKVCFHeaderLines.getFormatLine(GATKVCFConstants.HAPLOTYPE_CALLER_PHASING_GT_KEY));
headerLines.add(GATKVCFHeaderLines.getFormatLine(GATKVCFConstants.HAPLOTYPE_CALLER_PHASING_ID_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_RMS_MAPPING_QUALITY_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RMS_MAPPING_QUALITY_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_MAP_QUAL_RANK_SUM_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_MAP_QUAL_RANK_SUM_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_QUAL_APPROX_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.RAW_QUAL_APPROX_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_READ_POS_RANK_SUM_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_READ_POS_RANK_SUM_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_SB_TABLE_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.SB_TABLE_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_VQS_LOD_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_YNG_STATUS_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_VARIANT_DEPTH_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.VARIANT_DEPTH_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_STRAND_ODDS_RATIO_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_FISHER_STRAND_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_QUAL_BY_DEPTH_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.QUAL_BY_DEPTH_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.EXCESS_HET_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.SB_TABLE_KEY));

headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.NAY_FROM_YNG));
headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.EXCESS_HET_KEY));

headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.EXCESS_ALLELES));
headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.NO_HQ_GENOTYPES));

return headerLines;
}

public enum OutputType {
TSV,
ORC,
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -123,12 +123,6 @@ public static VCFHeaderLine getVqsLodHeader(Double vqsLodThreshold, String model
"Site failed " + model + " model VQSLOD cutoff of " + vqsLodThreshold.toString());
}

public static VCFHeaderLine getExcessAllelesHeader(int excessAllelesThreshold, String model) {
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Unreferenced header field

// should there be a gate for max or min excessAllelesThreshold value?
return new VCFFilterHeaderLine(GATKVCFConstants.VQSR_FAILURE_PREFIX + model,
"Site failed " + model + " model excess allele cutoff (" + excessAllelesThreshold + ")");
}

public static VCFHeaderLine getTruthSensitivityHeader(Double truthSensitivityThreshold, Double vqsLodThreshold, String model) {
if (truthSensitivityThreshold == null) { // at this point, we know that all vqsr threshold inputs are null, so use defaults
truthSensitivityThreshold = GATKVCFConstants.SNP.contains(model) ? DEFAULT_TRUTH_SENSITIVITY_THRESHOLD_SNPS : DEFAULT_TRUTH_SENSITIVITY_THRESHOLD_INDELS;
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -316,22 +316,22 @@ protected void onStartup() {
extraHeaderLines.add(FilterSensitivityTools.getTruthSensitivityHeader(truthSensitivityINDELThreshold, vqsLodINDELThreshold, GATKVCFConstants.INDEL));
}
} else {
extraHeaderLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_VQS_SENS_KEY));
extraHeaderLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.CALIBRATION_SENSITIVITY_KEY));
if (truthSensitivitySNPThreshold == null) {
truthSensitivitySNPThreshold = FilterSensitivityTools.DEFAULT_TRUTH_SENSITIVITY_THRESHOLD_SNPS;
}
truthSensitivitySNPThreshold /= 100.0;
logger.info("Passing all SNP variants with calibration_sensitivity < " + truthSensitivitySNPThreshold);
logger.info("Passing all SNP variants with " + GATKVCFConstants.CALIBRATION_SENSITIVITY_KEY + " < " + truthSensitivitySNPThreshold);

if (truthSensitivityINDELThreshold == null) {
truthSensitivityINDELThreshold = FilterSensitivityTools.DEFAULT_TRUTH_SENSITIVITY_THRESHOLD_INDELS;
}
truthSensitivityINDELThreshold /= 100.0;
logger.info("Passing all INDEL variants with calibration_sensitivity < " + truthSensitivityINDELThreshold);
logger.info("Passing all INDEL variants with " + GATKVCFConstants.CALIBRATION_SENSITIVITY_KEY + " < " + truthSensitivityINDELThreshold);

extraHeaderLines.add(new VCFFilterHeaderLine(GATKVCFConstants.VQS_SENS_FAILURE_SNP,
extraHeaderLines.add(new VCFFilterHeaderLine(GATKVCFConstants.CALIBRATION_SENSITIVITY_FAILURE_SNP,
"Site failed SNP model calibration sensitivity cutoff (" + truthSensitivitySNPThreshold.toString() + ")"));
extraHeaderLines.add(new VCFFilterHeaderLine(GATKVCFConstants.VQS_SENS_FAILURE_INDEL,
extraHeaderLines.add(new VCFFilterHeaderLine(GATKVCFConstants.CALIBRATION_SENSITIVITY_FAILURE_INDEL,
"Site failed INDEL model calibration sensitivity cutoff (" + truthSensitivityINDELThreshold.toString() + ")"));
}
}
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -32,15 +32,15 @@ String getVQScoreFieldName() {
}

String getAlleleSpecificVQSScoreKey() {
return GATKVCFConstants.AS_VQS_SENS_KEY;
return GATKVCFConstants.CALIBRATION_SENSITIVITY_KEY;
}

String getVqScoreSNPFailureFilterName() {
return GATKVCFConstants.VQS_SENS_FAILURE_SNP;
return GATKVCFConstants.CALIBRATION_SENSITIVITY_FAILURE_SNP;
}

String getVqScoreINDELFailureFilterName() {
return GATKVCFConstants.VQS_SENS_FAILURE_INDEL;
return GATKVCFConstants.CALIBRATION_SENSITIVITY_FAILURE_INDEL;
}

public ExtractCohortLiteEngine(final String projectID,
Expand Down
Original file line number Diff line number Diff line change
@@ -1,8 +1,11 @@
package org.broadinstitute.hellbender.tools.gvs.filtering;

import htsjdk.samtools.SAMSequenceDictionary;
import htsjdk.variant.variantcontext.writer.VariantContextWriter;
import htsjdk.variant.vcf.VCFConstants;
import htsjdk.variant.vcf.VCFHeader;
import htsjdk.variant.vcf.VCFHeaderLine;
import htsjdk.variant.vcf.VCFStandardHeaderLines;
import org.broadinstitute.barclay.argparser.Argument;
import org.broadinstitute.barclay.argparser.CommandLineProgramProperties;
import org.broadinstitute.barclay.help.DocumentedFeature;
Expand Down Expand Up @@ -136,12 +139,9 @@ protected void onStartup() {
sampleList = new SampleList(sampleTableName, sampleFileName, projectID, printDebugInformation, "extract-features");

Set<VCFHeaderLine> extraHeaderLines = new HashSet<>();
extraHeaderLines.add(
FilterSensitivityTools.getExcessAllelesHeader(excessAllelesThreshold, GATKVCFConstants.EXCESS_ALLELES));

extraHeaderLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.LOW_QUAL_FILTER_NAME));

VCFHeader header = CommonCode.generateVcfHeader(
VCFHeader header = generateVcfHeader(
new HashSet<>(), reference.getSequenceDictionary(), extraHeaderLines);

vcfWriter = createVCFWriter(IOUtils.getPath(outputVcfPathString));
Expand Down Expand Up @@ -216,4 +216,79 @@ protected void onShutdown() {
}
}

private static VCFHeader generateVcfHeader(Set<String> sampleNames,
final SAMSequenceDictionary sequenceDictionary,
final Set<VCFHeaderLine> extraHeaders) {
final Set<VCFHeaderLine> headerLines = new HashSet<>();

headerLines.addAll( getEvoquerVcfHeaderLines() );
headerLines.addAll( extraHeaders );
final VCFHeader header = new VCFHeader(headerLines, sampleNames);
header.setSequenceDictionary(sequenceDictionary);

return header;
}

private static Set<VCFHeaderLine> getEvoquerVcfHeaderLines() {
final Set<VCFHeaderLine> headerLines = new HashSet<>();
// Add standard VCF fields first:
VCFStandardHeaderLines.addStandardInfoLines( headerLines, true,
VCFConstants.STRAND_BIAS_KEY,
VCFConstants.DEPTH_KEY,
VCFConstants.RMS_MAPPING_QUALITY_KEY,
VCFConstants.ALLELE_COUNT_KEY,
VCFConstants.ALLELE_FREQUENCY_KEY,
VCFConstants.ALLELE_NUMBER_KEY,
VCFConstants.END_KEY
);

VCFStandardHeaderLines.addStandardFormatLines(headerLines, true,
VCFConstants.GENOTYPE_KEY,
VCFConstants.GENOTYPE_QUALITY_KEY,
VCFConstants.DEPTH_KEY,
VCFConstants.GENOTYPE_PL_KEY,
VCFConstants.GENOTYPE_ALLELE_DEPTHS
);

headerLines.add(GATKVCFHeaderLines.getFormatLine(GATKVCFConstants.REFERENCE_GENOTYPE_QUALITY));

headerLines.add(GATKVCFHeaderLines.getFormatLine(GATKVCFConstants.HAPLOTYPE_CALLER_PHASING_GT_KEY));
headerLines.add(GATKVCFHeaderLines.getFormatLine(GATKVCFConstants.HAPLOTYPE_CALLER_PHASING_ID_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_RMS_MAPPING_QUALITY_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RMS_MAPPING_QUALITY_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_MAP_QUAL_RANK_SUM_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_MAP_QUAL_RANK_SUM_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_QUAL_APPROX_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.RAW_QUAL_APPROX_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_RAW_READ_POS_RANK_SUM_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_READ_POS_RANK_SUM_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_SB_TABLE_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.SB_TABLE_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_VARIANT_DEPTH_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.VARIANT_DEPTH_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_STRAND_ODDS_RATIO_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_FISHER_STRAND_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.AS_QUAL_BY_DEPTH_KEY));
headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.QUAL_BY_DEPTH_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.EXCESS_HET_KEY));

headerLines.add(GATKVCFHeaderLines.getInfoLine(GATKVCFConstants.SB_TABLE_KEY));

headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.EXCESS_HET_KEY));
headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.EXCESS_ALLELES));
headerLines.add(GATKVCFHeaderLines.getFilterLine(GATKVCFConstants.NO_HQ_GENOTYPES));

return headerLines;
}

}
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