From 79f23800d90ab64eef060b09d9edd61a14e612b2 Mon Sep 17 00:00:00 2001 From: James Bonfield Date: Mon, 29 Jan 2024 16:50:25 +0000 Subject: [PATCH] Add SAM recommendation of MAPQ=0 for unmapped reads We already have a recommendation that MAPQ of 255 should not be used. Expand on this to recommend zero is used when unmapped. This is purely a recommendation, made for maximum compatibility, and not a specification requirement. Fixes #727 --- SAMv1.tex | 1 + 1 file changed, 1 insertion(+) diff --git a/SAMv1.tex b/SAMv1.tex index c1d76d1a..5470b23e 100644 --- a/SAMv1.tex +++ b/SAMv1.tex @@ -737,6 +737,7 @@ \section{Recommended Practice for the SAM Format} \item Unmapped reads should be stored in the orientation in which they came off the sequencing machine and have their {\sf reverse} flag bit~(0x10) correspondingly unset. + \item Unmapped reads should have mapping quality zero. \end{enumerate} \item Multiple mapping \begin{enumerate}[label=\arabic*]